About Calzyme Contact Us Locations Our Policies

  Welcome to Calzyme!

3-Phosphoglycerate Kinase (PGK)

(ATP:3-phospho-D-glycerate 1-phosphotransferase; EC
3-Phosphoglycerate kinase (PGK) catylizes the following reaction:
Glycerate-3-P + ATP {--------} Glycerate-1,3-P2 + ADP

PGK has a molecular weight of 47,000. This enzyme does not react with AMP, PEP or glycerate-2,3-P2.

The amount of enzyme required to convert 1 mM of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH

6.9 at 25°C.


1. 0.1 M Triethanolamine¿HCl-NaOH buffer, containing 2 mM MgCl 2 and 1 mM EDTA disodium, pH 7.5

2. 200 mM 3-phospho-D-glycerate

3. 100 mM ATP

4. 12 mM NADH in 10 mM Tris.

1. Set the spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.

2. Into a quartz cuvette pipette the following:

        Triethanolamine buffer                                                        2.60 ml

        3-phospho-D-glycerate                                                      0.10 ml

       ATP                                                                                 0.05 ml

       NADH/Tris                                                                        0.05 ml

3. Add 10 µl of GAPDH and incubate at 25°C for five minutes. Record blank rate at 340 nm.

4. Add 0.1 ml enzyme solution to the cuvette. Mix and record the increase in absorbance at 340 nm for 5 minutes.

5. Calculate ΔE340nm/min

Activity (U/mg) = (ΔE340nm/min)(Total Vol.)(Enz. Diln.)
(6.22)(Enz. Vol.)(mg Enz./ml)

©1999-2018 Calzyme All Rights Reserved powered by corezon
All logos and trademarks are properties of their respective companies. Product price, specification, warranty and availability subject to change without notice.
2-Phosphoenol Pyruvate Acid 3-Indoxyl Phosphate
3-Phosphoglycerate Kinase Acetone Powders
Acetyl Cholinesterase Acid Phosphatase
Actin Acylase
Adenosine 5'-diphosphate Adenosine 5'-monophosphate (AMP)
Adenosine 5'-triphosphate (ATP) Adenosine Deaminase
Alcohol Dehydrogenase Aldehyde Dehydrogenase
Aldolase Alkaline Phosphatase
alpha-Amylase alpha-D-Glucose-1-Phosphate
alpha-Glucosidase alpha-Ketoglutarate
Amylopectin Amylose
Apo-d-Amino Acid Oxidase Ascorbate Oxidase
Avidin beta-Amylase
beta-Glucosidase beta-Glucronidase
beta-NAD beta-NADH
beta-NADPH Bromelain
Butyryl Cholinesterase C-Reactive Protein
Carbonic Anhydrase Carboxypeptidase A
Carboxypeptidase B Carboxypeptidase Y
Catalase Catechol-o-Methyl Transferase
Cathepsin D Cholesterol Esterase
Chymotrypsin d-Amino Acid Oxidase
d-Glucose-6-Phosphate Deoxyribonuclease (DNase)
Deoxyribonucleic Acid (DNA) Elastase
Enterokinase Ferritin
G-6-phosphate Dehydrogenase gamma-Glutamyl Transferase
Glucose Oxidase Glutamate Dehydrogenase
Glutathione Peroxidase Glycerol Kinase
Glycerol-3-phosphate Dehydrogenase Glycosylated Albumin
Glycosylated Hemoglobin GOT (AST)
GPT (ALT) Hemoglobin
 Hexokinase Histamine-n-Methyl Transferase
Hyaluronic Acid Hyaluronidase
Insulin L-Arginase
L-gamma-Glutamide Nitroanilide Lactate Dehydrogenase
Lactoperoxidase Leucine Aminopeptidase
Lipase Lipoamide Dehydrogenase
Lipoxidase Luciferase
Lysozyme Malate Dehydrogenase
Mutarotase Myelin
Myoglobin Myosin
Oxyhemoglobin p-Nitrophenyl Phosphate
Pepsin Peroxidase
Phosphorylase-b Phspho(enol) Pyruvate Carboxylase
PNMT Prophospholipase A2
Protein Methylase II Pyruvate Kinase
Renin Rennin
Ribonuclease Sedoheptulose
Stachyose Superoxide Dismutase
Thyroglobulin Triose Phosphate Isomerase
Trypsin Trypsin Inhibitor
Tyrosinase Urease
Uricase Xanthine Oxidase