(N-Acylamino acid aminohydrolase; EC 188.8.131.52)
Acylase 1 (Amino Acylase 1) catalyzes the following reaction:
R1 CH2 COOH + NH2 R2 CHOOH
R1 = Cl, H, NH2 , etc.
R2 = L-Amino acid side chain other than L-aspartic acid
Acylase 1 is an extremely stable enzyme in the dry state. In solution, the enzyme is stable at high temperatures (70° C) at pH 7, but below pH 5 it is rapidly and irreversibly inactivated. The enzyme shows a high degree of optical specificity toward its substrates. For this reason, it has been used extensively for the resolution of racemic amino acids.