About Calzyme Contact Us Locations Our Policies

  Welcome to Calzyme!

Adenosine Deaminase

(Adenosine aminohydrolase; EC

Adenosine + H2O Inosine + NH3

The enzyme is widely distributed in animal tissues (E.J. Conway and R. Cooke, Biochem J., 33, 457-479, 1939). The highest enzyme activities are found in the mucosa of the small intestine, in the appendix and the spleen (V.D. Hoagland jr. and I.R. Fisher, J. Biol. Chem., 242, 4341, 1967) and (B.M Chassy and R.J. Suhadolnik, J. Biol. Chem., 242, 3655, 1967). The enzyme is present in the cytoplasmic cell fraction and also to some extent in the nucleus (R.M. Smillie, Arch. Biochem., 67, 213, 1957) and (H.K Jordan, et al., J. Neurochem., 4, 170, 1959). The optimum pH is 7.0 to 7.4.

1.Source: Calf Intestine
   Form: Freeze-dried powder 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at -20 C (-4 F) 
   Activity (approx.): 3 U/mg protein 
   Protein (approx.): 95% 
   Catalog No.: 212A0200 

One unit will deaminate 1.0 &mgr;mole of adenosine to inosine per min at pH 7.4 at 25° C.

1. 0.05 M Phosphate buffer, pH 7.4.
2. Adenosine, 3.6 mg/ml dissolved in buffer.
3. Enzyme solution in buffer (1-2 U/ml).

1. Set up spectrophotometer (with strip chart and temp. control) to 265 nm and 25° C.
2. Into a cuvette, pipette the following:
Phosphate buffer 2.97 ml
Adenosine 0.01 ml
Incubate cuvette in spectrophotometer at 25° C for 5 minutes to attain temperature equillibration and then establish blank rate if any. 3. Initiate reaction by adding 0.02 ml enzyme solution.
4. Calculate E265nm/min

Activity (U/mg) = (ΔE265nm/min)(Total Vol.)(Enz. Diln.)
(8.1)(Enz. Vol.)(mg Enz./ml)

©1999-2018 Calzyme All Rights Reserved powered by corezon
All logos and trademarks are properties of their respective companies. Product price, specification, warranty and availability subject to change without notice.
2-Phosphoenol Pyruvate Acid 3-Indoxyl Phosphate
3-Phosphoglycerate Kinase Acetone Powders
Acetyl Cholinesterase Acid Phosphatase
Actin Acylase
Adenosine 5'-diphosphate Adenosine 5'-monophosphate (AMP)
Adenosine 5'-triphosphate (ATP) Adenosine Deaminase
Alcohol Dehydrogenase Aldehyde Dehydrogenase
Aldolase Alkaline Phosphatase
alpha-Amylase alpha-D-Glucose-1-Phosphate
alpha-Glucosidase alpha-Ketoglutarate
Amylopectin Amylose
Apo-d-Amino Acid Oxidase Ascorbate Oxidase
Avidin beta-Amylase
beta-Glucosidase beta-Glucronidase
beta-NAD beta-NADH
beta-NADPH Bromelain
Butyryl Cholinesterase C-Reactive Protein
Carbonic Anhydrase Carboxypeptidase A
Carboxypeptidase B Carboxypeptidase Y
Catalase Catechol-o-Methyl Transferase
Cathepsin D Cholesterol Esterase
Chymotrypsin d-Amino Acid Oxidase
d-Glucose-6-Phosphate Deoxyribonuclease (DNase)
Deoxyribonucleic Acid (DNA) Elastase
Enterokinase Ferritin
G-6-phosphate Dehydrogenase gamma-Glutamyl Transferase
Glucose Oxidase Glutamate Dehydrogenase
Glutathione Peroxidase Glycerol Kinase
Glycerol-3-phosphate Dehydrogenase Glycosylated Albumin
Glycosylated Hemoglobin GOT (AST)
GPT (ALT) Hemoglobin
 Hexokinase Histamine-n-Methyl Transferase
Hyaluronic Acid Hyaluronidase
Insulin L-Arginase
L-gamma-Glutamide Nitroanilide Lactate Dehydrogenase
Lactoperoxidase Leucine Aminopeptidase
Lipase Lipoamide Dehydrogenase
Lipoxidase Luciferase
Lysozyme Malate Dehydrogenase
Mutarotase Myelin
Myoglobin Myosin
Oxyhemoglobin p-Nitrophenyl Phosphate
Pepsin Peroxidase
Phosphorylase-b Phspho(enol) Pyruvate Carboxylase
PNMT Prophospholipase A2
Protein Methylase II Pyruvate Kinase
Renin Rennin
Ribonuclease Sedoheptulose
Stachyose Superoxide Dismutase
Thyroglobulin Triose Phosphate Isomerase
Trypsin Trypsin Inhibitor
Tyrosinase Urease
Uricase Xanthine Oxidase