(D-Fructose-1, 6-diphosphate-D-glyceraldehyde-3-phosphate-lyase; EC 18.104.22.168)
Aldolase catalyzes the following key reaction in glycolysis:
D-Fructose-1,6 diphosphate <-------> Dihydroxyacetone phosphate + D-glyceraldehyde-3-phosphate
Aldolase is found in all animal and plant tissues and most
microorganisms. In animal tissues, three types of aldolases
have been identified: Type A, the major form found in muscle;
Type B found in kidney and liver and Type C found in
brain. The enzyme may exist in five different isoenzyme forms
which may be organ-specific.
Aldolase from rabbit muscle has been extensively studied. It
has a molecular weight of 161,000 and consists of four
catalytically active sub-units.
Measurement of serum aldolase activity is of clinical
significance. Elevated serum aldolase activity has been
observed in certain carcinomas, muscular dystrophy, hepatitis
and myocardial infarction (Kaldor, J., Clin. Chim. Acta, 28,
145, 1970). Aldolase is also used for determination of
metabolites, in coupled enzyme reactions. The is 9.38 and
isoelectric point is 6.1. It is inhibited by heavy metal
ions, like copper, silver and zinc.