About Calzyme Contact Us Locations Our Policies

  Welcome to Calzyme!

Butyryl Cholinesterase (BChE)

(Acylcholine acylhydrolase; EC

Butyryl cholinesterase catalyzes the hydrolysis of a number of choline esters as shown:

Acylcholine + H2O ---------> Choline + Corresponding Acid
The enzyme is sometimes referred to as ÒpseudocholinesteraseÓ but it preferentially uses butyrylcholine and benzoylcholine as substrates. Butyrylcholinesterase is distinct from acetylcholinesterase and is found in mammalian blood plasma, liver, pancreas, intestinal mucosa and the white matter of the central nervous system.

Butyrylcholinesterase from equine serum has a molecular weight of 440,000 (Lee, J.C. and Harpst, J.A., Biochemistry, 12, 1622, l973). It is a glycoprotein which has an optimum pH of 6.0-8.0. Assay of butyrylcholinesterase activity is of diagnostic value in various liver diseases, malignancies, and pulmonary tuberculosis (Methods of Enzymatic Analysis; Bergmeyer, H.U. ed: Vol. 2, 833, 1974. Academic Press, New York.) The enzyme can also be used for the assay of organophosphorous compounds such as pesticides.

The amount of enzyme causing the hydrolysis of one micromole of butyrylthiocholine iodide per minute at 25°C and pH 7.4.

  1. 0.05 M Tris-HCl buffer, pH 7.4.
  2. 0.25 mM 51,51-Dithiobis [2-nitrobenzoic aci] (DTNB) 0.1 mg/ml in 0.05 M Tris-HCl buffer, pH 7.4.
  3. 0.111 mM Butyrylthiocholine iodide (24 mg/ml in glass distilled water)
  4. Enzyme solution in 0.05 M Tris-HCl buffer, pH 7.4., to yield a concentration of 0.1 U/ml.

  1. Set spectrophotometer (equipped with strip chart recorder and temperature control) at 405 nm and 25°C.
  2. Into cuvettes, place the following reagents:
    DTNB 2.70 ml
    Butyrylthiocholine iodide 0.20 ml

    Mix and incubate in spectrophotometer for 5 min. and establish blank rate, if any.

  3. Initiate the reaction by adding 0.1 ml of the enzyme. Record the rate of absorbance at 405 nm for 5 min.
  4. Calculate (delta)E405 nm/min

Activity (U/mg) = (ΔE405nm/min)(Enz. Diln.)
(13.3)(Enz. Vol.)(mg Enz./ml)

©1999-2018 Calzyme All Rights Reserved powered by corezon
All logos and trademarks are properties of their respective companies. Product price, specification, warranty and availability subject to change without notice.
2-Phosphoenol Pyruvate Acid 3-Indoxyl Phosphate
3-Phosphoglycerate Kinase Acetone Powders
Acetyl Cholinesterase Acid Phosphatase
Actin Acylase
Adenosine 5'-diphosphate Adenosine 5'-monophosphate (AMP)
Adenosine 5'-triphosphate (ATP) Adenosine Deaminase
Alcohol Dehydrogenase Aldehyde Dehydrogenase
Aldolase Alkaline Phosphatase
alpha-Amylase alpha-D-Glucose-1-Phosphate
alpha-Glucosidase alpha-Ketoglutarate
Amylopectin Amylose
Apo-d-Amino Acid Oxidase Ascorbate Oxidase
Avidin beta-Amylase
beta-Glucosidase beta-Glucronidase
beta-NAD beta-NADH
beta-NADPH Bromelain
Butyryl Cholinesterase C-Reactive Protein
Carbonic Anhydrase Carboxypeptidase A
Carboxypeptidase B Carboxypeptidase Y
Catalase Catechol-o-Methyl Transferase
Cathepsin D Cholesterol Esterase
Chymotrypsin d-Amino Acid Oxidase
d-Glucose-6-Phosphate Deoxyribonuclease (DNase)
Deoxyribonucleic Acid (DNA) Elastase
Enterokinase Ferritin
G-6-phosphate Dehydrogenase gamma-Glutamyl Transferase
Glucose Oxidase Glutamate Dehydrogenase
Glutathione Peroxidase Glycerol Kinase
Glycerol-3-phosphate Dehydrogenase Glycosylated Albumin
Glycosylated Hemoglobin GOT (AST)
GPT (ALT) Hemoglobin
 Hexokinase Histamine-n-Methyl Transferase
Hyaluronic Acid Hyaluronidase
Insulin L-Arginase
L-gamma-Glutamide Nitroanilide Lactate Dehydrogenase
Lactoperoxidase Leucine Aminopeptidase
Lipase Lipoamide Dehydrogenase
Lipoxidase Luciferase
Lysozyme Malate Dehydrogenase
Mutarotase Myelin
Myoglobin Myosin
Oxyhemoglobin p-Nitrophenyl Phosphate
Pepsin Peroxidase
Phosphorylase-b Phspho(enol) Pyruvate Carboxylase
PNMT Prophospholipase A2
Protein Methylase II Pyruvate Kinase
Renin Rennin
Ribonuclease Sedoheptulose
Stachyose Superoxide Dismutase
Thyroglobulin Triose Phosphate Isomerase
Trypsin Trypsin Inhibitor
Tyrosinase Urease
Uricase Xanthine Oxidase