About Calzyme Contact Us Locations Our Policies

  Welcome to Calzyme!

Carboxypeptidase Y

(Peptidyl-L-amino-acid (-L-proline ) hydrolase; EC

Carboxypeptidase Y (CPY) catalyzes the following reaction:

 Peptidyl-L-amino acid + H2O ------> Peptide + L-amino acid
It is prepared according to the method of Moore & Stein (J. Biol Chem, 211, 907, 1954). It resembles Carboxypeptidase A in its substrate specificity, but it hydrolyzes C-terminal glycine and L-leuicine more rapidly and L-phenylalanine more slowly.

One unit of enzyme activity is defined as that amount of enzyme that catalyzes the hydrolysis of 1 micromole of substrate per minute.

Carboxypeptidase Y activity is determined from the increase in absorbance due to the hydrolysis of hippuryl-L-arginine at 254 nm.

  1. 50 mM Sodium phosphate buffer (pH 6.5)
  2. 1 mM Benzyloxycarbonyl-phenylalanyl-L-leucine
  3. Enzyme solution: dilute enzyme with distilled water to a concentration of 1-5 U/ml.

  1. Set the water bath at 25C. Pipette 1.0 ml of substrate solution into a test tube:1mM Benzyloxycarbonlyl-phenylalanyl-L-leucine in 50mM sodium phosphate buffer, pH 6.5.
  2. Incubate at 25C for 15 minutes.
  3. Mix in 50 l of CPY solution diluted to around 0.3 U/ml and let the reaction procede for 15 min.
  4. Stop the reaction by adding1.0 ml of ninhydrin reagent and heat for 20 minutes in boiling water.
  5. Add 5.0 ml of 50% ethanol solution.
  6. Measure (delta)E570 against a blank, zero time sample.

Activity (U/mg) = (ΔE570nm/min)(Total Vol.)(Enz. Diln.)
(Extin. Coeff of ninhydrine)(10 min.)(Enz. Vol.)

©1999-2018 Calzyme All Rights Reserved powered by corezon
All logos and trademarks are properties of their respective companies. Product price, specification, warranty and availability subject to change without notice.
2-Phosphoenol Pyruvate Acid 3-Indoxyl Phosphate
3-Phosphoglycerate Kinase Acetone Powders
Acetyl Cholinesterase Acid Phosphatase
Actin Acylase
Adenosine 5'-diphosphate Adenosine 5'-monophosphate (AMP)
Adenosine 5'-triphosphate (ATP) Adenosine Deaminase
Alcohol Dehydrogenase Aldehyde Dehydrogenase
Aldolase Alkaline Phosphatase
alpha-Amylase alpha-D-Glucose-1-Phosphate
alpha-Glucosidase alpha-Ketoglutarate
Amylopectin Amylose
Apo-d-Amino Acid Oxidase Ascorbate Oxidase
Avidin beta-Amylase
beta-Glucosidase beta-Glucronidase
beta-NAD beta-NADH
beta-NADPH Bromelain
Butyryl Cholinesterase C-Reactive Protein
Carbonic Anhydrase Carboxypeptidase A
Carboxypeptidase B Carboxypeptidase Y
Catalase Catechol-o-Methyl Transferase
Cathepsin D Cholesterol Esterase
Chymotrypsin d-Amino Acid Oxidase
d-Glucose-6-Phosphate Deoxyribonuclease (DNase)
Deoxyribonucleic Acid (DNA) Elastase
Enterokinase Ferritin
G-6-phosphate Dehydrogenase gamma-Glutamyl Transferase
Glucose Oxidase Glutamate Dehydrogenase
Glutathione Peroxidase Glycerol Kinase
Glycerol-3-phosphate Dehydrogenase Glycosylated Albumin
Glycosylated Hemoglobin GOT (AST)
GPT (ALT) Hemoglobin
 Hexokinase Histamine-n-Methyl Transferase
Hyaluronic Acid Hyaluronidase
Insulin L-Arginase
L-gamma-Glutamide Nitroanilide Lactate Dehydrogenase
Lactoperoxidase Leucine Aminopeptidase
Lipase Lipoamide Dehydrogenase
Lipoxidase Luciferase
Lysozyme Malate Dehydrogenase
Mutarotase Myelin
Myoglobin Myosin
Oxyhemoglobin p-Nitrophenyl Phosphate
Pepsin Peroxidase
Phosphorylase-b Phspho(enol) Pyruvate Carboxylase
PNMT Prophospholipase A2
Protein Methylase II Pyruvate Kinase
Renin Rennin
Ribonuclease Sedoheptulose
Stachyose Superoxide Dismutase
Thyroglobulin Triose Phosphate Isomerase
Trypsin Trypsin Inhibitor
Tyrosinase Urease
Uricase Xanthine Oxidase