About Calzyme Contact Us Locations Our Policies

  Welcome to Calzyme!

Catalase

(H2O2: H2O2 oxidoreductase; EC 1.11.1.6)

Catalase is a heme-containing enzyme which catalyzes the following reaction:

     CATALASE
2H2O2 -------------------> 2H2O + O2
Catalase is present in a wide variety of tissues from animals, plants and microorganisms. Mammalian liver contains high concentrations of the enzyme. Bovine liver catalase has a molecular weight of 250,000 with four subunits of equal size. Optimum pH is 7.0 and isoelectric point is 5.4.

Encapsulated or immobilized catalase is used in the food industry whenever hydrogen peroxide needs to be destroyed, for example, in the manufacture of cheese (Chu, H.D., Leeder, J.G. and S.C. Gilbert, J. Food Sci, 40, 641, 1975). Catalase can also be used in coupled systems for the determination of metabolites in biological fluids.



1.Source: Aspergillus Niger
   Form: Freeze-dried powder 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at -20 C (-4 F) 
   Activity: 2000 U/mg protein 
   Protein: 95% 
   Catalog No.: 191A2000 
$1.00/KU
2.Source: Bovine Liver
   Form: Freeze-dried powder 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at -20 C (-4 F) 
   Activity: 5,000 U/mg protein 
   Protein: 95% 
   Catalog No.: 194A5000 
$10.00/MU


The amount of enzyme which catalyzes the decomposition of one micromole of H2O2 per minute at 25C and pH 7.00.


The decomposition of hydrogen peroxide, which is a measure of catalase activity, can be followed by measuring the absorbance at 240 nm (Methods of Enzymatic Analysis, Bergmeyer, H.U., ed., Vol 1, p 438, 1974, Academic Press, New York).


  1. 0.05 M Potassium phosphate buffer, pH 7.0.
  2. 0.05% Hydrogen Peroxide (substrate) solution. Dilute 0.2 ml of 30% H2O2 to 100 ml with phosphate buffer. The absorbance at 240 nm should be in the range of 0.50-0.55.
  3. Catalase (enzyme) solution. Dilute in buffer to yield a concentration of 5-10 U/ml. Prepare fresh prior to assay.



  1. Set spectrophotometer (equipped with a strip chart recorder and temperature control) at 240 nm and 25C.
  2. In a quartz cuvette pipette 2.9 ml of diluted H2O2 solution (substrate). Incubate in spectrophotometer at 25C for 5 min. to attain temperature equilibration. Record absorbance at 240 nm (blank).
  3. Initiate reaction by adding 0.1 ml diluted enzyme (catalase) solution to the cuvette. Record decrease in absorbance at 240 nm for 2-3 minutes.
  4. Calculate (delta)E240 nm/min



Activity (U/mg) = (ΔE240nm/min)(Total Vol.)(Enz. Diln.)
(0.043)(Enz. Vol.)(mg Enz./ml)
 

©1999-2017 Calzyme All Rights Reserved powered by corezon
All logos and trademarks are properties of their respective companies. Product price, specification, warranty and availability subject to change without notice.
2-Phosphoenol Pyruvate Acid 3-Indoxyl Phosphate
3-Phosphoglycerate Kinase Acetone Powders
Acetyl Cholinesterase Acid Phosphatase
Actin Acylase
Adenosine 5'-diphosphate Adenosine 5'-monophosphate (AMP)
Adenosine 5'-triphosphate (ATP) Adenosine Deaminase
Alcohol Dehydrogenase Aldehyde Dehydrogenase
Aldolase Alkaline Phosphatase
alpha-Amylase alpha-D-Glucose-1-Phosphate
alpha-Glucosidase alpha-Ketoglutarate
Amylopectin Amylose
Apo-d-Amino Acid Oxidase Ascorbate Oxidase
Avidin beta-Amylase
beta-Glucosidase beta-Glucronidase
beta-NAD beta-NADH
beta-NADPH Bromelain
Butyryl Cholinesterase C-Reactive Protein
Carbonic Anhydrase Carboxypeptidase A
Carboxypeptidase B Carboxypeptidase Y
Catalase Catechol-o-Methyl Transferase
Cathepsin D Cholesterol Esterase
Chymotrypsin d-Amino Acid Oxidase
d-Glucose-6-Phosphate Deoxyribonuclease (DNase)
Deoxyribonucleic Acid (DNA) Elastase
Enterokinase Ferritin
G-6-phosphate Dehydrogenase gamma-Glutamyl Transferase
Glucose Oxidase Glutamate Dehydrogenase
Glutathione Peroxidase Glycerol Kinase
Glycerol-3-phosphate Dehydrogenase Glycosylated Albumin
Glycosylated Hemoglobin GOT (AST)
GPT (ALT) Hemoglobin
 Hexokinase Histamine-n-Methyl Transferase
Hyaluronic Acid Hyaluronidase
Insulin L-Arginase
L-gamma-Glutamide Nitroanilide Lactate Dehydrogenase
Lactoperoxidase Leucine Aminopeptidase
Lipase Lipoamide Dehydrogenase
Lipoxidase Luciferase
Lysozyme Malate Dehydrogenase
Mutarotase Myelin
Myoglobin Myosin
Oxyhemoglobin p-Nitrophenyl Phosphate
Pepsin Peroxidase
Phosphorylase-b Phspho(enol) Pyruvate Carboxylase
PNMT Prophospholipase A2
Protein Methylase II Pyruvate Kinase
Renin Rennin
Ribonuclease Sedoheptulose
Stachyose Superoxide Dismutase
Thyroglobulin Triose Phosphate Isomerase
Trypsin Trypsin Inhibitor
Tyrosinase Urease
Uricase Xanthine Oxidase


abc