(H2O2: H2O2 oxidoreductase; EC 220.127.116.11)
Catalase is a heme-containing enzyme which catalyzes the
2H2O2 -------------------> 2H2O + O2
Catalase is present in a wide variety of tissues from animals,
plants and microorganisms. Mammalian liver contains high
concentrations of the enzyme. Bovine liver catalase has a
molecular weight of 250,000 with four subunits of equal size.
Optimum pH is 7.0 and isoelectric point is 5.4.
Encapsulated or immobilized catalase is used in the food
industry whenever hydrogen peroxide needs to be destroyed, for
example, in the manufacture of cheese (Chu, H.D., Leeder,
J.G. and S.C. Gilbert, J. Food Sci, 40, 641, 1975). Catalase
can also be used in coupled systems for the determination of
metabolites in biological fluids.