(Peptidyl peptide hydrolase; EC 18.104.22.168)
Chymotrypsin occurs in the pancreas of mammals, and has been
isolated from bovine pancreas. It is an endopeptidase, which
preferentially splits peptide bonds in which the carbonyl
group is contributed by aromatic amino acids such as tyrosine,
phenylalanine, or tryptophan. In addition, chymotrypsin
catalyzes the hydrolysis of the bonds of esters and amides of
aromatic amino acids as well as proteins and peptides.
Chymotrypsin occurs in different forms which can be isolated
by electrophoresis. Different forms of chymotrypsin have
different degrees of catalytic activity.
Chymotrypsin catalyzes the following reaction:
N-Benzoyl-L-Tyrosine Ethyl Ester + H2O ------> N-Benzoyl-L-Tyrosine + Ethanol