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(Pancreato peptidase E; EC

Elastase is a unique proteolytic enzyme in mammals because of its ability to hydrolyze the insoluble protein elastin. In addition, it also exhibits endopeptidase activity on other proteins. Elastase is produced in the pancreas and it also occurs in leukocytes and in the serum. It has been postulated that elastase activity may play role in the induction of atherosclerosis and acute hemorrhagic pancreatitis (Bieth, J., Spiess, B. and C.G. Wermuth, Biochem. Med., 11, 350, 1974).

Elastase from porcine pancreas has been extensively studied. It has a molecular weight of 25,900 (Shotton, D.M. and B.S. Hartley, Biochem J., 131, 643, 1973). Elastase can be used for degradation of proteins and peptides.

1.Source: Porcine Pancreas
   Form: Freeze-dried powder 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at -20 C (-4 F) 
   Activity (approx.): 10 U/mg protein 
   Purity (approx.): 90% (biuret) 
   Catalog No.: 075A0010 
2.Source: Porcine Pancreas
   Form: Water suspension 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at 4 C (39 F); Do not freeze 
   Activity (approx.): 5 U/mg protein 
   Purity (approx.): 90% (biuret) 
   Catalog No.: 075D0005 

The amount of enzyme which hydrolyzes one micromole of SucAla3Na per minute at 25C, pH 8.0.

The assay method is described by Feinstein, et al, (Feinstein, G., Kupfer, A., and M. Slklolvsky, Biochem Biophys Res. Comm., 50, 1020, 1973).

  1. 0.1 M Tris buffer, pH 8.0.
  2. 0.0044 M SucAla3Na substrate dissolved in Tris buffer (2 mg/ml).
  3. 0.15 NaCl

  1. Set spectrophotometer (equipped with a strip chart recorder and temperature control) at 410 nm and 25C.
  2. Pipette into a cuvette:
    Tris Buffer 2.7 ml
    Diluted elastase 0.1 ml
    Mix and incubate in a shaker-bath at 37C for 4-5 min.
  3. To test cuvette add 0.2 ml of substrate, mix and record increase in A410 for 5 minutes.
  4. Calculate (delta)A/min from the linear protion standard curve.

Activity (U/mg) = (ΔA410nm/min)(Total Vol.)(Enz. Diln.)
(8.8)(mg Enz./ml)

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