(L-Glutamate:NAD(P)+ Oxidoreductase; EC 188.8.131.52)
Glutamate dehydrogenase (GLDH) catalyzes the following
L-glutamate + NAD+ + H2O ------> a-ketoglutarate + NADH + NH4+
The enzyme can also use NADP+ as electron acceptor. Glutamate
dehydrogenase plays a significant role in deamination of amino
acids especially in those organisms that excrete
ammonia. Glutamate dehydrogenase from rat and beef liver has
been extensively studied. The enzyme from beef liver has a
molecular weight of 280,000.
The reaction catalyzed by glutamate dehydrogenase can be used
clinically in determination of ammonia and products which can
be converted to ammonia in body fluids such as blood urea
nitrogen (BUN) determination. This enzyme can also be utilized
in the assay of GOT and GPT.