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Glycosylated Hemoglobin

Glycosylated hemoglobin is the name given to hemoglobin that has been modified by reaction with glucose or similar derivative of this sugar. As blood sugar levels are higher in diabetic patients than in normal individuals, the amount of glycosylated hemoglobin in diabetics can reach four times the value found under normal circumstances and thus the measurement of glycosylated hemoglobin can be used to assess how well the condition is being kept under control (Gonen, et al, The Lancet, 734-737, 1977 ). The glycosylated hemoglobin that is found in blood consists of a varity of products, the major one being HbAlc (Rahbar, et al, Biochem. Biophys. Res. Comm., 36, 838-843, 1969). Studies on this derivative indicate that the sugar may exist as a stable ketimine or as an unstable aldimine, the conversion of one form to the other being achieved by an Amadori rearrangement. In HbAlc the glucose is attached to the N-terminal valine of the beta-chain.


1.Source: Human Hemoglobin
   Form: Freeze-dried powder 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at -20 C (-4 F) 
   Purity: Free of unreacted hemoglobin 
   Catalog No.: 163A0000 
$100.00/mg


The glycosylated hemoglobin is assayed by electrophoresis on agar gels following the method of Menard et al, Clin. Chem., 26,1598-1602, 1980. This measurement gives the percent of glycosylated hemoglobin with respect to total hemoglobin in the sample. A further measurement by electrophoresis on agarose gels gives the percent hemoglobin with respect to total protein in the sample.
 

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