We define the purity in terms of a preparative definition. The highly polymerized nature of the material is established in terms of the limiting viscosity number [h]-the level of which exceeds for all grades.
Protein determinations are carried out by the method of Lowry (Lowry, A.L., et al, J. Biol. Chem., 193, 265, 1951). Chondroitin sulfate is detected on electrophoresis on cellulose acetate using Alcian Blue as dye. The homogeneity of Hyaluronic acid is further confirmed by chromatography on DEAE-cellulose (Schmidt, M., Biochim. Biophys. Acta, 63, 346, 1962; Berman, E.R., Biochim. Biophys. Acta, 58, 120, 1962).