About Calzyme Contact Us Locations Our Policies

  Welcome to Calzyme!

Ribonuclease (RNase)

(Ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC

Pancreatic ribonucluease (RNase) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of an adjacent pyrimidine nucleotide forming 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. RNase is found in greatest quantity in ruminant pancreas (Barnard, E.A., 'Biological Functions of Pancreatic Ribonuclease', Nature, 240, 340, 1969) and has been extensively studied. The major component is RNase A and the minor component is RNase B which is a glycoprotein (Beintema, J.J.,et al, Eur J. Biochem., 63, 441, 1976) and have molecular weights of 13,700 and 14,700 respectfully.

The amount of enzyme which causes an increase in absorbance of 1.0 at 260 nm at 37°C and pH 5.0.

The method of Kalnitsky (Kalnitsky, et al, J. Biol. Chem., 234, 1512, 1959) is used to measure the amount of acid soluble oligonucleotide liberated under the given conditions.

  1. 0.10 M Sodium acetate buffer, pH 5.0
  2. 25% Perchloric acid containing 0.75% Uranyl acetate
  3. Enzyme: 1% yeast RNA in 0.10 M sodium acetate, pH 5.0. Preincubate at 37°C for 5-8 minutes prior to assay.

  1. Prepare stock solution at 1 mg/ml in water. Immediately prior to assay, dilute further to 2, 4 and 6 µg/ml in 0.10 M sodium acetate.
  2. Pipette 1 ml of respective enzymes dilution into centrifuge tubes. Include a blank containing 1 ml of 0.10 M sodium acetate buffer, pH 5.0.
  3. Incubate all tubes at 37°C for 5-8 minutes.
  4. At zero time and timed intervals add 1 ml of 1% RNA and incubate each tube exactly 4 minutes.
  5. Stop reaction by adding 1 ml of uranyl acetate-perchloric acid solution.
  6. Transfer to an ice bath and cool for 5 minutes.
  7. Clarify by centrifugation and dilute 0.1 ml of clear supernatant to 3.0 ml with water.
  8. Read E260 against the blank.

Activity (U/mg) = (ΔE260nm/min)(Total Vol.)(Enz. Diln.)
(Enz. Vol.)(mg Enz./ml)

©1999-2018 Calzyme All Rights Reserved powered by corezon
All logos and trademarks are properties of their respective companies. Product price, specification, warranty and availability subject to change without notice.
2-Phosphoenol Pyruvate Acid 3-Indoxyl Phosphate
3-Phosphoglycerate Kinase Acetone Powders
Acetyl Cholinesterase Acid Phosphatase
Actin Acylase
Adenosine 5'-diphosphate Adenosine 5'-monophosphate (AMP)
Adenosine 5'-triphosphate (ATP) Adenosine Deaminase
Alcohol Dehydrogenase Aldehyde Dehydrogenase
Aldolase Alkaline Phosphatase
alpha-Amylase alpha-D-Glucose-1-Phosphate
alpha-Glucosidase alpha-Ketoglutarate
Amylopectin Amylose
Apo-d-Amino Acid Oxidase Ascorbate Oxidase
Avidin beta-Amylase
beta-Glucosidase beta-Glucronidase
beta-NAD beta-NADH
beta-NADPH Bromelain
Butyryl Cholinesterase C-Reactive Protein
Carbonic Anhydrase Carboxypeptidase A
Carboxypeptidase B Carboxypeptidase Y
Catalase Catechol-o-Methyl Transferase
Cathepsin D Cholesterol Esterase
Chymotrypsin d-Amino Acid Oxidase
d-Glucose-6-Phosphate Deoxyribonuclease (DNase)
Deoxyribonucleic Acid (DNA) Elastase
Enterokinase Ferritin
G-6-phosphate Dehydrogenase gamma-Glutamyl Transferase
Glucose Oxidase Glutamate Dehydrogenase
Glutathione Peroxidase Glycerol Kinase
Glycerol-3-phosphate Dehydrogenase Glycosylated Albumin
Glycosylated Hemoglobin GOT (AST)
GPT (ALT) Hemoglobin
 Hexokinase Histamine-n-Methyl Transferase
Hyaluronic Acid Hyaluronidase
Insulin L-Arginase
L-gamma-Glutamide Nitroanilide Lactate Dehydrogenase
Lactoperoxidase Leucine Aminopeptidase
Lipase Lipoamide Dehydrogenase
Lipoxidase Luciferase
Lysozyme Malate Dehydrogenase
Mutarotase Myelin
Myoglobin Myosin
Oxyhemoglobin p-Nitrophenyl Phosphate
Pepsin Peroxidase
Phosphorylase-b Phspho(enol) Pyruvate Carboxylase
PNMT Prophospholipase A2
Protein Methylase II Pyruvate Kinase
Renin Rennin
Ribonuclease Sedoheptulose
Stachyose Superoxide Dismutase
Thyroglobulin Triose Phosphate Isomerase
Trypsin Trypsin Inhibitor
Tyrosinase Urease
Uricase Xanthine Oxidase